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发明授权
CN100593025C 一种制备β-淀粉样多肽1-42纯品的方法 有权

一种制备β-淀粉样多肽1-42纯品的方法
摘要：
本发明涉及一种多肽的制备方法，特别涉及一种利用高效液相色谱制备β-淀粉样多肽1-42纯品的方法。以解决现有高效液相色谱方法进行制备存在的设备要求高、使用的流动相体系比较繁琐的技术问题。其制备条件为：色谱柱：PLRP-S，10-15μm，21.2×250mm(I.D)，流动相：A：0.1-0.2%三氟乙酸水，B：0.1-0.2%三氟乙酸乙腈，0到25分钟A∶B由90-70∶10-30到10-30∶90-70，流速：15ml/min，波长：220nm，样品浓度：100-200mg/10ml，上样量：100-200mg。本发明所述的制备方法，使用的流动相是在高效液相色谱中非常常用的试剂，除了高效液相色谱仪不再需要别的仪器，既节省成本又能够简单、快速地得到需要的纯品。
摘要（英）：

The invention relates to a polypeptide preparation method, in particular to a method for using high-efficiency liquid chromatography to prepare beta-amyloid 1-42 pure product, to resolve the technicalproblems of prior high-efficiency liquid chromatography as high equipment demands and complex flowing system. The preparation conditions comprise that PLRP-S, 10-15um, 21.2x250mm(I.D), flowing phase:A: 0.1-0.2% trifluoroacetic acid water, B: 0.1-0.2% trifluoroacetic acid acetonitrile, 0-25min, A:B is from 90-70:10-30 to 10-30:90-70, flowing speed is 15ml/min, wavelength is 220nm, sample densityis 100-200mg/10ml and sample amount is 100-200mg. The preparation method uses general agent of high-efficiency liquid chromatography as flowing phase and eliminates other machines beside high-efficiency liquid chromatography machine, to save cost and simply obtain pure product quickly.

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