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发明公开
CN101008016A 为转基因克隆动物提供高表达细胞核的方法 无效 - 驳回

为转基因克隆动物提供高表达细胞核的方法
摘要：
本发明提供一种为转基因克隆动物提供高表达细胞核的方法，解决载体中乳腺启动子难以在成纤维细胞中发挥作用，并且由此而产生的难以鉴别外源基因表达水平的问题；主要是采取细胞融合方法，用转染人生长激素基因的鼠胚成纤维细胞与具备分泌功能的人乳腺癌细胞株MCF－7/S细胞相融合，融合细胞具备了乳腺启动子(βLG)发挥作用的条件，使融合细胞分泌人生长激素；本发明暂时避开了一系列有待解决的理论问题，在融合后放免分析中即可筛选出在转基因细胞水平上表达量较高的细胞克隆作为核供体，简便易行，大大节省人力物力。
摘要（英）：

The invention provides a method for offering high- expressed nucleus to transgenic clonic animal, and solves problem of difficulty of detecting external gene expression level caused by inactive lactiferous gland promotor in desmocyte. It mainly takes cell fusion method, fusing mouse embryo desmocyte transfected with human adenohypophyseal growth hormone gene and human mammary gland cancer cell strain MCF-7/S with secretory function, the fused cell possesses condition for activating mammary gland promotor (beta LG) and the fused cell will secrete human growth hormone. The invention avoids a serial of pending theorial questions, and can sift out cell colony with high expression amount on transgenic cell level which used as nuclear donator in radioimmunoassay after fusion.

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