The present invention discloses one kind of composite litchi-male silk moth nutrient liquid and its preparation process. The composite litchi-male silk moth nutrient liquid is prepared with litchi juice in 1-50 wt%, male silk moth in 0.01-10 wt% and proper amount of pure water; and its preparation process includes the steps of milling male silk moth in a colloidal mill while adding water, adding protease in 0.01- 0.1 wt% of dry male silk moth for enzymolysis under the optimal conditions, cold storing the enzymolyzed liquid at 4 deg.c for 2-5 hr, centrifuging, mixing the supernatant with litchi juice, regulating pH value to over 5, adding salt, sugar and stabilizer, homogenizing, sterilizing and packing. The composite litchi-male silk moth nutrient liquid, which may have other fruit juice added, can provide human body with minerals, vitamins, amino acids and other nutritious components, and has health functions of refreshing, delaying senility, etc.