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发明公开
CN101068934A 用于反复合成寡核苷酸的组合物、方法及检测技术 无效 - 撤回

用于反复合成寡核苷酸的组合物、方法及检测技术
摘要：
本发明提供用于检测目的分子的存在的方法，所述的方法通过在多核苷酸序列上反复进行酶促寡核苷酸合成事件产生多重可检测的寡核苷酸，并检测所述的产物。本发明也提供用于多重无效转录的方法。在进一步的方面，本发明提供含有无效启动子盒的树状聚合物的用途。在一方面，本发明提供适合用于本发明的无效启动子盒。在一方面，无效转录的产物通过使用质谱法检测。在另一方面，本发明提供用于检测靶蛋白、DNA或RNA的方法，所述的方法通过用无效转录产生多重可检测的RNA寡核糖核苷酸，所述的RNA寡核糖核苷酸可以例如通过质谱法检测。
摘要（英）：

The present invention provides methods for detecting the presence of a molecule of interest by generating multiple detectable oligonucleotides through reiterative enzymatic oligonucleotide synthesis events on a polynucleotide sequence and detecting said products. The invention also provides for methods of multiplex abortive transcription. In a further aspect, the invention provides for the use of dendrimers containing abortive promoter cassettes. In one aspect, the invention provides for abortive promoter cassettes suitable for use in the present invention. In one aspect the products of abortive transcription are detected with the use of mass spectrometry. In another aspect, the invention provides a method for detecting a target protein, DNA or RNA by generating multiple detectable RNA oligoribonucleotides by abortive transcription that are detected, including by mass spectrometry.

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