The present invention relates to a single direction valve used in the micro invasive surgical puncture device of the laparoscopy and the thoracoscopy. In the present invention, the improved single direction valve used in the puncture is arranged in the cavity of the sheath tube tail of the puncture. The single direction valve comprises a V-typed funnel structure. a bottom plate provided with a cutting opening for the puncture rod and a supporting body, which provides sealing pressure to the cutting opening. The supporting structure is connected with the outer part of the bell mouth in the V-typed funnel structure. Symmetric supporting gluten is arranged between the inner part of the supporting body and the inclined plate of the V-typed funnel structure. The supporting gluten distributes in both sides of the cutting opening. Auxiliary gluten with a certain thickness and vertical to the cutting opening is arranged in the prominent part of the outer wall in the supporting body. The single direction valve of the present invention has deliberate structure and convenient manufacture as well as high reliability. The sealing effect during application is excellent. The problem that the cutting opening can be winded towards the pulling direction (teeming laps) when the puncture rod is pulled out from the cutting opening of the single valve is resolved.