The invention discloses a cell electric physiology integrated chip and a manufacturing method. An MEA microelectrode array is integrated in the middle of the front face of the Si basis, an IDA (interdigital electrode) and a LAPS (light addressable potentionmetric sensor) are integrated on two sides of the MEA microelectrode array respectively, two reference electrodes are symmetrically arranged on the other two sides of the MEA microelectrode array. The integrated chip can detect the adherent growth state of the cell on the device, and the chip can detect the concentration of a plurality of ions of the metabolic productions and the action potential. The invention is used for realizing a simultaneous detection of multi-parameter, and the nucleus technique difficulties of the design and theprocessing of the integrated cell sensor chip are solved, and the invention expands a new application area for the portability and instrumentation development of the integrated cell sensor technique.