The invention discloses a sample nondestructive approximation method regarding nanometer observation and operation. A sample is controlled to do nanometer level initial adjustment movement relative to a probe and to do nanometer level precise movement. After undergoing the feedback control step of checking position variation signal of a reflected laser spot, the aim of controlling the sample to approach the probe without damage is achieved through carrying out feedback control of checking the photoelectric detection signal produced when the sample approaches the probe and generates an atomic force action. The invention can avoid probe or sample damage caused by collision approximation.