The invention adopts the femtosecond laser two-photon induced photo knife effect and the high photon density induced optical tweezers gradient force function for carrying out a micro-nano manipulation, which is applicable to the study of the injury, regeneration and repair of various biological cells. The invention consists of a laser generation system, an external optical path system, a high precision imaging system and a software control system. The laser generation system includes two parallel branches, one branch consists of a lock mould knob and a femtosecond laser sequentially, and the other branch consists of a digital delay/pulse signal generator and a regeneration amplifier sequentially. The external optical path system consists of a dual-branch optical path, a semi-transparent semi-reflection mirror, an optical gate and a beam expander sequentially. The dual-branch optical path includes two parts: one part consists of a movable convex lens, a fixed convex lens, a rotatable total reflection mirror and an attenuation mirror sequentially, and the other part is an attenuation mirror. The device can realize the whole process of nano-injury and regeneration study of the nerve cells and can adopt the femtosecond optical tweezers to realize the choice of the super-sophisticated target position during an axon regeneration process.