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发明公开
CN101246126A 沙门氏菌恒温基因扩增快速检测试剂盒及方法 无效 - 撤回

沙门氏菌恒温基因扩增快速检测试剂盒及方法
摘要：
本发明公开了一种沙门氏菌恒温基因扩增快速检测试剂盒及方法，试剂盒包括引物混合液、Bst DNA聚合酶、反应液、样品预处理液、显色剂、阳性对照及阴性对照，其中引物序列如SEQ ID NO.1、SEQ ID NO.2、SEQ ID NO.3、SEQ ID NO.4。利用该试剂盒通过提取沙门氏菌基因组DNA、恒温基因扩增反应、判断反应产物结果实现对沙门氏菌高特异性、快速、高灵敏度、简便的检测。
摘要（英）：

The present invention discloses a salmonella constant-temperature gene amplification fast detecting reagent kit and the method thereof, the reagent kit comprises the primer mixed liquid, the Bst DNA polyase, the reacting liquid, the sample pretreating liquid, the color developing agent, the positive comparison and negative comparison, wherein the sequence of the primer is the sequence such as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. Through extracting the salmonella genome DNA, constant-temperature gene amplification reaction and judging the reaction product result with the reagent kit the high-specificity, quick, high-sensitivity and simple detecting to the salmonella is realized.

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