The invention relates to a method for separating bio-oil through high boiling point solvent, solving the problem that the prior method for separating different components of bio-oil pollutes the environment. The method comprises the following steps that: glycerin is used as a solvent and a heat transfer medium; in the temperature range between 200 and 250 DEG C, bio-oil is distilled under atmospheric pressure so as to obtain fractions; residual glycerin mixture is mixed with water to separate out lignin precipitates; the glycerin mixture mixed with water is heated to 230 DEG C to recover crude glycerin and water to be recycled. The method can integrate bio-oil separation with the prior petrochemical process, so as to obtain organic components and cracking lignin with different boiling points, completely avoids the use of inorganic acid, alkali and volatile organic solvent, avoids pollution, improve the environmental friendly property and safety of separation process. The method is simple in technological operation and easy to realize. As the glycerin and water are recycled and the needed heat energy is provided by the bio-oil, the economic cost of the separation process is reduced.