The invention discloses a glutamic acid extraction process, which comprises heating glutamic acid fermenting liquor, ultra-filtering, and filtering with a nano-filter system, heating the fermenting liquid, adjusting the pH value to 3.0-4.0 with sulfuric acid, concentrating by evaporation in a crystallizer pan; and discharging to a crystallization promoting tank when 50%-80% of water is evaporated off, slowly cooling to a temperature below 60 DEC C, and centrifugally separating glutamic acid. The glutamic acid extraction process has small usage amount of acid and alkali, less waste water amount, and higher extraction efficiency of the glutamic acid. The obtained glutamic acid is ensured that the product quality reaches the national standard.