The invention discloses a method for preparing phosphatidyl laurinol by enzymatic methods, which belongs to the phospholipid enzymatic method modification technical field. Soybean mixed phospholipid is dissolved into anhydrous organic solvent, laurinol is added to perform the base exchange reaction under the catalysis of treated peanut phosphatidase D, and the mixed phospholipid is transformed into the phosphatidyl laurinol. The reactant passes through a wash organic phase, is subject to the dehydration and exsolution, and is completely scrubbed by acetone, undissolved substances of the acetone are separated, and a product is obtained after the drying. The content of the phosphatidyl laurinol of the product is more than 65 percent through HPLC determination. No water exists in the method,so the product does not contain compositions which are not expected to contain in phosphatidyl laurinol products such as lysophosphatide and phosphatidic acid and so on; and the method can conveniently prepare the phosphatidyl laurinol which has broad use in the pharmaceutical industry and liposome industry.