The present invention provides a ZnO nanorod array detection kit which is used for early diagnosis of the liver cancer. The kit adopts anti-AFP protein, anti-AFP (alpha-fetoprotein) protein, anti-sugar protein 19-9 and anti-sugar protein 125; the kit consists of an improved ZnO nanorod array film which is coated by the four kinds of protein, a bottom plate, a micro field device (with an electrophoresis tank and a pair of electrodes) and buffer solution; the invention also relates to the utilizing and detecting method of the kit. The kit can be used for the diagnosis of benign and malignant primary liver cancer tumors, in particular for the detection of the early clinical primary liver cancer, and can be used for the chemotherapeutic monitoring and the prognostic evaluation after the operation on the liver cancer. The invention has the characteristics of high speed, sensitivity, accuracy, reliability, simple operation, and suitability for the primary health care units and the hospitals of various levels.