The invention discloses an ampoule medicine box used for a rescue vehicle and a manufacture method thereof. The invention relates to the technical field of medical apparatus. The ampoule medicine boxused for a rescue vehicle consists of a medicine box body and lattices in the medicine box body used for arranging a plurality of ampoules. The invention is characterized in that the lattice is a medicine lattice which consists of a transverse clapboard and a longitudinal clapboard which are mutually crossed and arranged; then the medicine lattice is arranged in the medicine box body; besides, thetransverse clapboard inclines by 45 degrees relative to the bottom surface of the medicine box body; a plurality of ampoule slots used for arranging the ampoules are arranged in parallel in the transverse clapboards of each medicine lattice; the ampoule slots are matched with the shapes of injection ampoules; the ampoule slot consists of an ampoule neck slot, an ampoule body and an ampoule block;a card slot is arranged above the ampoule slot of the transverse clapboard in the medicine lattice. The ampoule medicine box leads the storage of the injection ampoules to be safe and reliable; the rescue medicines in the medicine lattices are arranged in order, thus being convenient for the identifying, taking, complementing and checking of the rescue medicines.