The invention provides a test structure used for cutting a fuse wire with laser, which comprises a first fuse wire and a second fuse wire, wherein, the first fuse wire and the second fuse wire are arrayed by interval; the first fuse wire is electrically connected to form a serial fuse link in series; the first fuse wire and the second fuse wire are electrically connected to form a fuse link in parallel. The test structure also comprises a first electrode pad, a second electrode pad and a third electrode pad, wherein, the first electrode pad and the second electrode pad are electrically connected to the two ends of the fuse link in series respectively, and the third electrode pad is electrically connected to one end of the fuse link in parallel, which is not connected to the first electrodepad and the second electrode pad. By using the test structure to test the cutting fuse wire with laser, the effect of cutting the fuse wire with laser can be predicted, so the parameters of an opticalmaser can be adjusted to generate the laser to cut the fuse wire precisely. The invention also discloses a testing method for cutting the fuse wire with laser, a method for cutting the fuse wire with laser and a test wafer.