The invention discloses a method for preparing a freeze-dried live attenuated hepatitis A vaccine by utilizing a cell factory. The method has good process controllability, is uneasy to pollute environment, is suitable for industrialized and mass production, improves the utilization rate of a culture space by more than 3 times, ensures that the growth density of cells reaches between 4.0x10<6> and 5.0x10<6> cells per ml, assures the multiplication space of viruses, and also effectively solves the problem of cell residue after the cell factory digests through methods such as sterile washing, centrifugal treatment and so on, so as to continuously use the cell factory for cell culture and improve the reuse rate of the cell factory.