The invention relates to a technique for operating a nanometer-sized object in the nanometer science and technology and in particularly discloses an SPM probe reinforcing operating method. A start position and a termination positioned are determined according to control parameters; and the number of probe operation points is calculated and operations of translation and rotation are realized, according to a single-step operation distance or rotation angle, an operation point distance and an operation direction, which are regulated in a translation operation programming method and a rotation operation programming method based on probe preprogramming. The adoption of the invention can overcome the defect that due to the small diameter of the probe, only point operation can be performed to anoperated object, but effective control to a posture of the operated object is impossible. The invention also has the advantages of small step pitch, fast multi-point orientation, highly efficient operation and operated object posture control.