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发明公开
CN101423834A 肠毒素C2超抗原突变蛋白基因的克隆和异源表达方法 无效 - 撤回

肠毒素C2超抗原突变蛋白基因的克隆和异源表达方法
摘要：
本发明涉及基因工程技术，具体说是肠毒素C2超抗原突变蛋白基因及克隆表达和制备方法。具体为具有序列表SEQ ID NO：1、SEQ ID NO：2、SEQID NO：3和SEQ IDNO：4中碱基序列。本发明基因的异源表达提供了肠毒素C2在抗肿瘤方面的开发潜力，本发明在SEC2的基础上对其氨基端进行了删除突变，其氨基端对其生物活性的影响，同时在此基础上希望获得保留超抗原活性的突变蛋白。其制备超抗原抗肿瘤生物制剂，具有产量高，生产稳定，方便纯化的特点。
摘要（英）：

The invention relates to a genetic engineering technology, in particular to a superantigen mutant protein gene of enterotoxin C2 and a method for cloning, expressing and preparing the same. The superantigen mutant protein gene of the enterotoxin C2 comprises base sequences in tables of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3 and SEQ ID NO: 4. Allogenetic expression of the superantigen mutant protein gene provides development potential of the enterotoxin C2 in the anti-tumor aspect; and the superantigen mutant protein gene performs deletion mutant on an amino terminal thereof on the basis of SEC2, the amino terminal affects the biological activity, and mutant protein kept with the superantigen activity is expected to be obtained on the basis simultaneously. The superantigen mutant protein gene of the enterotoxin C2 applied to preparing a superantigen anti-tumor biological agent has the characteristics of high yield, stable production, convenient purification and the like.

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