The invention relates to mineral leaching bacteria and a biological high-temperature heap leaching technique of native copper sulfide ore that uses the leaching bacteria. The leaching bacteria are preserved in China Center for Type Culture Collection in Wuhan University, and the preservation numbers are respectively CCTCC NO of M207062, M207063, M207064 and M206029. The heap leaching technique roughly smashes the native copper sulfide ore, finely smashes the native copper sulfide ore, and preprocesses the native copper sulfide ore by spraying a bacteria-containing solution, thus obtaining oreparticles that are suitable for biological leaching. The ore particles are processed by working procedures of stacking, heap leaching and spraying, leaching liquid recycling, copper-rich liquid extracting, back extracting and electrodeposition and the like, thus obtaining marketable cathode copper. The heap leaching process adopts a method that includes room temperature bacteria, medium thermophiles and extreme thermophiles and realizes the bacteria-containing spraying operation in phases, thus gradually raising the internal stack temperature. Finally the stock dump temperature is preserved between 10 DEG C and 80 DEG C, pH value is preserved between 1.2 and 1.8, and proper Fe concentration is beneficial to preserve the activity of the mineral leaching bacteria. The biological high-temperature heap leaching technique can completely utilize the low-grade native copper sulfide ore resources in old mines, raises the synthetic mine utilization level, saves cost and increases profits. The biological high-temperature heap leaching technique of native copper sulfide ore that uses the leaching bacteria is applicable to existing domestic low-grade native copper sulfide ore of old mines andthe copper ore resource exploit in remote areas of western highlands that mainly aims at exploiting native copper sulfide ore.