The invention belongs to the in vitro diagnostic reagent field, and particularly relates to a culture medium for identifying vancomycin-resistant Staphylococcus aureus. The culture medium not only contains nutrient substances, solidified material and miscellaneous bacteria inhibitor, but also contains vancomycin, as well as color indicator 5-bromo-4-chloro-3-indolyl-phosphate and nitroblue tetrazolium. The miscellaneous bacteria inhibitor can inhibit the growth of miscellaneous bacteria out of the Staphylococcus aureus, the vancomycin can inhibit the Staphylococcus aureus which is sensitive to the vancomycin, and the indicator can cause the vancomycin-resistant Staphylococcus aureus colony to show purple for being further differentiated from other drug-resistant bacteria. The invention combines the layers of screening culture of the vancomycin-resistant Staphylococcus aureus with the test of the characteristic enzyme thereof, can accurately and visually detect and identify the vancomycin-resistant Staphylococcus aureus, has strong operability, is suitable to be promoted in hospitals and other research institutions, and can help to guide the doctor for medicine use and develop novel antibiotics.