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CN101457210B 一种中性植酸酶PHYMJ11及其基因和应用 有权 转让

一种中性植酸酶PHYMJ11及其基因和应用
摘要：
本发明涉及基因工程领域，具体地，本发明涉及一种土地杆菌Pedobacter sp.MJ11，其保藏号为CGMCC No.2503，以及从其中得到的中性植酸酶PHYMJ11及其基因、包含该基因的重组载体和应用。本发明提供了一种中性植酸酶PHYMJ11，其氨基酸序列如SEQ ID NO.1所示，且本发明提供了编码上述中性植酸酶的基因phyMJ11。本发明获得一种中性植酸酶，其最适pH值为7.0，同时在pH5～9的范围内均具有高的酶活性。其在中性条件下水解豆粕中植酸磷的能力优于目前应用的植酸酶，这些性质使其能应用于淡水鱼类饲料中。
摘要（英）：

The invention relates to the genetic engineering field, especially to a land bacillus Pedobacter sp. MJ11 with a preservation number of CGMCC No. 2503 and a neutral phytase PHYMJ11, a gene thereof, a recombinant vector containing the same and its application. The invention provides the neutral phytase PHYMJ11 with an amino acid sequence shown as SEQ ID NO. 1 and a gene phyMJ11 for coding said neutral phytase. The neutral phytase obtained in the invention has the optimum pH value 7. 0 and simultaneously has a high enzymatic activity in a range of pH5-9. The ability for hydrolyzing a phytate phosphorus in a bean pulp of the phytase in a neutral condition is better than the present used phytase, which enables the phytase in the invention to apply to a fresh water fish feed stuff.

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