The present invention provides a hyperthermophilic lipase showing activity at high temperature condition and its coding gene, simultaneously provides a method for preparing a lipase by a recombinant bacterial strain, and application of a purified lipase in phenyl ethyl alcohol chiral separation. The amino acid sequence of the hyperthermophilic lipase has a homology more than 90as amino acid sequence shown as SEQ ID NO. 2. The beneficial effects of the invention are mainly show as follows: the invention provides the hyperthermophilic lipase with a stereoselectivity and its coding gene, the lipase has a good tolerance and a high selectivity capable of preparing a optically pure R-phenyl ethyl alcohol by selective hydrolysis of racemic phenethyl ester with a production rate more than 45% ande.e.P about 99%.