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Invention Publication
CN101509008A 乙肝病毒L基因和hGM-CSF的融合基因及其表达载体的构建与应用 无效 - 驳回

乙肝病毒L基因和hGM-CSF的融合基因及其表达载体的构建与应用
Abstract:
本发明公开了一种乙型肝炎病毒全包膜蛋白L基因与hGM－CSF基因的融合基因及其表达载体的构建与应用。该融合基因的真核表达载体pVAX1－L－hGM的构建和表达：设计扩增乙肝病毒全包膜蛋白L基因的1对引物和扩增hGM－CSF基因的1对引物；重组质粒pVL的构建；重组质粒pVAX1－L－hGM的构建；重组质粒pVAX1－L－hGM转染真核细胞及体外表达鉴定。该融合基因的酵母表达载体的构建和转化：设计扩增融合抗原基因L－linker－GM的1对引物；以质粒PVAX1－L－GM为模板扩增L－linker－GM；pPICZαC－L－GM酵母表达载体的构建；pPICZαC－L－GM酵母表达载体的转化。
Abstract(English):

The invention provides a fusion gene of a hepatitis B virus total envelope protein L gene and hGM-CSF gene, and construction and application of an expression vector thereof. Construction and expression of the eukaryotic expression vector pVAX1-l-hGM of the fusion gene comprise the following steps: design of a pair of primers amplifying the hepatitis B virus total envelope protein L gene and a pair of primers amplifying hGM-CSF gene; construction of a recombinant plasmid pVL; construction of a recombinant plasmid pVAX1-L-hGM; and transfection by a recombinant plasmid pVAX-L-hGM to an eukaryotic cell and in-vitro expression identification. Construction and transfer of a yeast expression vector of the fusion gene comprise design of a pair of primers amplifying a chimeric antigen gene L-linker-GM; amplification of L-linker-GM by using plasmid PVAX1-L-GM as a template; construction of a pPICZalphaC-L-GM yeast expression vector; and transfer of the pPICZalphaC-L-GM yeast expression vector.

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