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发明公开
CN101509009A 乙肝L基因和结核Ag85B融合基因及其表达载体的构建与应用 无效 - 驳回

乙肝L基因和结核Ag85B融合基因及其表达载体的构建与应用
摘要：
本发明公开了一种乙肝病毒全包膜L基因与结核杆菌Ag85B的融合抗原基因及其构建和表达方法。该融合基因的真核表达载体pVLA的构建和表达：根据乙肝病毒全包膜L基因设计1对扩增乙肝病毒全包膜L基因的引物，根据结核杆菌Ag85B基因的编码序列设计1对扩增Ag85B基因的引物；重组质粒pVL的构建；重组质粒pVLA的构建；重组质粒pVLA转染真核细胞及体外表达鉴定。本发明融合抗原基因及其真核表达载体和表达蛋白质均可用于制备乙肝和结核的联合DNA疫苗。
摘要（英）：

The invention discloses a fusion gene of a hepatitis B virus total envelope L gene and mycobacterium tuberculosis Ag85B and a constructing and expressing method thereof. Construction and expression of a eukaryotic expression vector pVLA of the chimeric antigen gene comprise design of a pair of primers amplifying the hepatitis B virus total envelope L gene according to the hepatitis B virus total envelope L gene; construction of a recombinant plasmid pVL; construction of a recombinant plasmid pVLA; and transfection by a recombinant plasmid pVLA to an eukaryotic cell and in-vitro expression identification. The chimeric antigen gene, the eukaryotic expression vector thereof and an expression protein can be used for preparing combined DNA vaccine of hepatitis B and tuberculosis.

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