The invention discloses a humanized neutralizing engineered antibody for targeting avian influenza virus H5N1, which is derived from Chinese antibody recombination library of highly pathogenic avian influenza H5N1 convalescent and the main characteristics are as follows: the recombinant antibody is determined by specific gene sequences of complementarity determining regions (CDRs) being present in the antibody light-chain and heavy-chain gene variable region, and obtains effectively-expressed neutralizing functional antibody of specific binding AIVHA (avian influenza virus hemagglutinin) protein HA from prokaryotic and eukaryotic cells. The utilization of the antibody CDR or part of or whole gene can remodel and produce various forms of engineered antibodies in procaryon, yeast, insect and eucaryon cells, and any expression systems, and can prevent or treat related diseases of H5N1 avian influenza virus clinically.