The present invention discloses a new plant tissue specificity expression promoter-GIF1 promoter and use thereof, wherein the promoter is separated from the crop grain filling gene GIF1. The invention simultaneously discloses a carrier comprising the promoter sequence and a host cell thereof. The invention also discloses the use of the promoter. The promoter is especially useful for guiding the tissue specificity expression in the plant, especially the specificity expression of the seed, internode and/or root of the plant thereby improving the quality of the plant (especially the crop).