The invention discloses a TcO nucleus marked ciprofloxacin dithiocarbamate complex and a preparation method and applications thereof. The complex uses the TcO nucleus as a central nucleus; and four sulphur atoms in two CPFXDTC ligand molecules generate complexation reaction with Tc to obtain a TcO-CPFXDTC complex. The complex has the advantages of high radiochemical purity, good stability, high intussusception of inflammation, rational target-to-nontarget ratio of inflammation to muscle, and low intussusception of liver, lung, spleen and the like. Therefore, the complex is a new developer of inflammation, with popularization and application values.