The invention relates to a screening method of degrading bacteria strains by taking DDVP as a substrate, which belongs to the technical field of pesticide residue after biodegradation. The method comprises the following steps: the bacteria strain samples are inoculated in an enrichment medium containing 100-150 ml of DDVP for switchover culture, then the domesticated liquid cultured in the step 1is taken and diluted for 10<4>-10<5>, then the diluent is coated on a beef extract-peptone solid flat plate, and then the bacteria strains are placed upside down at the constant temperature of 30-35 DEG C and cultured for 3-4 days; and the bacteria strains growing in the step 2 are streaked on a selective inorganic salt culture medium solid flat plate containing the DDVP as a sole carbon source and then cultivated for 4-5 days at the temperature of 30-35 DEG C and the growing bacteria strains are the degrading bacteria strains taking the DDVP as the sole carbon source. The provided screening method of the degrading bacteria strains by taking DDVP as the substrate can be used for screening the degrading bacteria strains for other organophosphorus insecticides and provides a certain theoretical guidance on the screening aspect of the pesticide residue degrading bacteria strains.