The invention relates to parts of a bicycle or a motorcycle, in particular to a connection structure between a rim using a tubeless tyre and a spoke and a manufacture method thereof. The connection structure between the rim using the tubeless tyre and the spoke comprises the rim, the spoke, an aluminum component, a sealing component and a copper head. The rim comprises a steel wire surface and a tyre bead surface; holes are formed in the steel wire surface, and the tyre bead surface has no holes; the connection structure further comprises a hollow rivet nut, the end part of the rivet nut is provided with a nut head protruding radially, the rivet nut is arranged in the holes on the steel wire surface, the nut head is arranged at the outer side of the hole in the steel wire surface, the rivet nut is provided with a radial flange ring at the inner side of the hole, the flange ring is tightly contacted with the inner wall of the steel wire surface, the rivet nut is internally provided with an internal screw which is matched with an external screw of the aluminum component, the aluminum component is internally provided with the sealing component and the copper head, and the spoke is connected with the copper head. The invention reduces the thickness and the weight of the steel wire surface of the rim on the basis of guaranteeing the tightness, simplifies a production process and reduces cost.