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发明公开
CN101649349A 食品中李斯特氏菌属微生物检测试剂盒及检测方法 失效 - 权利终止

食品中李斯特氏菌属微生物检测试剂盒及检测方法
摘要：
食品中李斯特氏菌属微生物检测试剂盒及检测方法，该试剂盒中包括一种检测溶液，该检测溶液中含10mM Tris·Cl、50mM KCl、25mM MgCl 2 、dNTP各2.5mM、Taq DNA聚合酶5U/μL及李斯特氏菌引物对10μM。本发明采用mPCR结合部分变性条件下的DHPLC技术，针对食品中李斯特氏菌属微生物进行灵敏便捷的检测，并建立应用于该方法的快速检测试剂盒。使用本发明的检测试剂盒及检测方法，可以高灵敏度地检测食品中李斯特氏菌属微生物，并且检侧耗时短，操作简捷，可以节省大量的劳力和财力，适合快速检测的要求。
摘要（英）：

The invention provides a Listeria microbial detection kit in food and a detection method thereof. The kit comprises a detection solution containing 10mM Tris.Cl, 50mM KCl, 25mM MgCl2, 2.5mM dNTP, 5U/muL of Taq DNA polymerase and 10mu M of Listeria primer. By adopting mPCR to combine with DHPLC technology under partial denaturation condition, the invention carries out sensitive and convenient detection on Listeria microbe in food and establishes a rapid detection kit applied in the method. By using the detection kit and the detection method in the invention, Listeria microbe in food can be detected with high sensitivity, which has short detection time, convenient operation, can save a large number of labor and financial resources, and can be suitable for the requirement of rapid detection.

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