The invention discloses an enzymolysis method of sea cucumber, comprising the following steps: a. pulping and homogenizing fresh sea cucumber to prepare sea cucumber pulp; b. directly adding papain in the sea cucumber pulp and ensuring that the specific activity of the papain in the sea cucumber pulp is 2,400-8,000mu/g and even stirring; and c. placing the sea cucumber pulp containing the papain in a boiling water bath, continuously stirring and hydrolyzing for 15-30 minutes. The method has the characteristics of energy saving, quickness and high efficiency; and the enzymolysis process and enzyme killing process are simultaneously finished, the obtained sea cucumber enzymolysis liquid has high hydrolysis degree and the hydrolysis degree of the sea cucumber protein subjected to the hydrolysis can reach about 85-95 percent.