The invention belongs to the field of medicinal preparation, relating to a temperature responsive bio-adhesive in situ gel sustained-release preparation for vagina drug administration. The preparation is composed of poloxamer, carrageenan, at least one optional gel substrate material, such as polyacrylic acid and the like and at least one drug with effective treating amount. The preparation combines the reversed-phase gelation properties of poloxamer and the bio-adhesive property and antiviral activity of carrageenan, and can obviously improve the sustained-release effect of the gel and can maintain effective drug concentration for above 12 hours in the vagina in vivio. The preparation has the advantages of convenient drug administration and favourable sustained release and is suitable for treating diseases of female reproductive system, in particular to sexually transmitted sex microorganism infection.