The invention discloses a method for preparing an emulsified medicinal composition containing oleum fructus bruceae, which comprises the following steps: (1) mixing a proper amount of water and 1 to 10ml of glycerin; (2) adding at least one of yolk lecithin and poloxamer into liquid prepared in step (1), and performing shear mixing uniformly, wherein the yolk lecithin is between 0.5 and 10g, and the poloxamer is between 0.1 and 5g; and (3) slowly adding 5 to 30ml of oleum fructus bruceae into liquid prepared in step (2), adding water to make the total content be 100ml, adjusting the pressure of a high pressure homogenizer, adopting two-step emulsification method, and continuously circulating 3 to 6 times to prepare the emulsified medicinal composition containing the oleum fructus bruceae.The oleum fructus bruceae emulsion injection prepared by the method has good stability, no hemolysis in a period of validity, and safety, and is favorable for clinical promotion and application.