The invention aims to solve the technical problem that the existing method for testing the replication capacity of hepatitis B virus (HBV) and the sensitivity of antiviral drugs has defects of complicated steps and accurate result. The technical scheme for solving the problem is to provide a primer group of 1.3 copies of HBV genome which comprises primer pairs used for preparing HBV2.4 section through amplification and primer pairs used for performing amplification to HBV1.7 section. Meanwhile, the invention provides a kit containing the above primer group and a method for testing the replication capacity of hepatitis B virus and the sensitivity of antiviral drugs. The method of the invention utilizes the structural features of HBV genomic sequence and creatively adopts the two-step amplification method to obtain 1.3 copies of HBV genome, the process is simple, the cycle is short, the cost is low and the detects of the prior art are well overcome, thus the method has good application prospect.