The invention provides a method for detecting the release of an anaphylactic medium in a Chinese medicament injection, which is a method for measuring the trace secretion of the anaphylactic medium namely histamine, capable of causing human body anaphylactic reactions, in the Chinese medicament injection. The method comprises the following steps of: adopting an ethyl chloroformate derivatization method; performing qualitative and quantitative analysis on the histamine by using a GC-MS detection method; and performing determination by analyzing a plurality of important parameters during measuring. Test results show that a chromatogram map obtained by the method has a smooth baseline and a good peak form, and the method is simple, convenient, quick and sensitive, has good repeatability, and can effectively detect the endogenous released histamine caused by the Chinese medicament injection.