The invention relates to an anti-bacterial thermoplastic composite, which comprises the following mixed components: 100 parts of thermoplastic by weight and 0.1-40 parts of composite antibacterial agent by weight, wherein the composite antibacterial agent comprises micronano inorganic/organic particles and guanidinesalt polymers which are coated on the surfaces of the micro/nano particles and/or embedded among pores and layers of the micronano particles; the weight ratio of the guanidinesalt polymers to the micro/nano particles is 1:1-1:50; and the micro/nano particles have at least a one-dimensional average size of 1nm-1000mum. The anti-bacterial thermoplastic composite is obtained by melting and mixing the components such as the thermoplastic, the composite antibacterial agent, and the like, therefore the antibacterial rate of the anti-bacterial thermoplastic composite is higher and the preparation method is simple.