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发明授权
CN101724707B 食品中四种致敏原麸质成分实时荧光PCR检测引物和探针、试剂盒及检测方法 失效 - 权利终止 转让

食品中四种致敏原麸质成分实时荧光PCR检测引物和探针、试剂盒及检测方法
摘要：
本发明涉及食品中四种致敏原麸质成分实时荧光PCR检测引物和探针、试剂盒及检测方法。该引物和探针分别是序列为SEQ ID NO.1～3的大麦Hordein基因的检测引物和探针、序列为SEQ ID NO.4～6的小麦Gliadin基因的检测引物和探针、序列为SEQ ID NO.7～9的黑麦Sec1基因的检测引物和探针和序列为SEQ ID NO.10～12的燕麦Avenin基因的检测引物和探针。基于此，本发明公开了包含上述引物和探针序列的食品致敏原麸质成分实时荧光PCR检测试剂盒及相应的检测方法。本发明的探针和引物特异性强，检测试剂盒及方法简便易用，结果准确，具有很高的特异性和灵敏度。
摘要（英）：

The invention relates to a primer, a probe and a kit of detecting real-time fluorescence PCR of four allergen gluten components in foods and a detection method. The primer and the probe are respectively a detection primer and a detection probe of barley Hordein genes with sequences of SEQ ID NO.1-3, barley Gliadin genes with sequences of SEQ ID NO.4-6, rye Secl genes with sequences of SEQ ID NO.7-9 and oat Avenin genes with sequences of SEQ ID NO.10-12. Based on the condition, the invention discloses the kit for detecting the real-time fluorescence PCR of the food allergen gluten components and the corresponding detection method. The probe and the primer have strong specificity, and the detection kit and the method have simple and easy use, accurate result and high specificity and flexibility.

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