The invention discloses an F-embedded termite drug and a eparation method and application thereof. The preparation method comprises the following steps: (1) slk fibroin and N-amber Asian amide in 1:100 mass ratio are dissolved in 1-fluorine-2,4-dinitrobenzene and PBS, and are mixed and react in ice bath, to form optical-activity silk fibroin solution; (2) the optical-activity silk fibroin solution is dripped on the surface of clean material, a termite drug is added in, and another layer of optical-activity silk fibroin solution is paved on to be cooled, dried, embedded, radiated by ultraviolet light for 15 to 20min, so that the embedded drug is grafted to the material, and the SF-embedded termite drug is prepared. The SF-embedded termite drug prepared by the method of the invention has the effect of long-time effect and releasing, improves the toxic effect to the termite, and reduces the pollution caused by the termite drug to the environment.