The invention relates to a method for determining a mine single-row curtain-grouting hole distance, which belongs to the technical field of mining and is used for solving the problems in reasonably determining the curtain-grouting hole distance. The method comprises the following steps: a, determining the thickness T of the curtain; b, determining the permeability q0 of the curtain wall; c, determining the active diffusion radius R of the grout; d, determining the distance D0 between every two grouting points on the same level; and e, determining and calculating the grouting hole distance D. The invention breaks through the construction convention that the grouting hole distance in the curtain engineering is 10m, combines the various parameters of curtain grouting, and adopts an experiment and theory combination method to determine the reasonable grouting hole distance for curtain grouting, thereby achieving the best grouting water-plugging effect with reasonable engineering investment. The experiment indicates that when the grouting is carried out according to the hole distance determined by the method, the water permeability of the inspection holes can meet the requirements and is qualified on quality detection. The invention ensures the effect of the curtain, saves the investment cost, shortens the construction period and has favorable economic benefit.