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发明公开
CN101793785A 甲醛测定试剂(盒)及甲醛浓度测定方法 无效 - 撤回

甲醛测定试剂(盒)及甲醛浓度测定方法
摘要：
本发明涉及一种利用酶倍增法、酶比色法及酶联法技术的甲醛测定试剂(盒)，同时本发明还涉及测定甲醛浓度的方法、试剂的组成及成分，属于食品/环境检验测定技术领域。本发明的试剂(盒)主要成分包括：缓冲液、辅酶、乙酰-辅酶A、谷胱甘肽、甲醛脱氢酶、甲酸C-乙酰基转移酶、辅酶A-谷胱甘肽还原酶及稳定剂；通过将样品与试剂按一定的体积比混合，使之发生一系列的酶促反应，再将反应物置于紫外/可见光分析仪下，检测主波长340nm处吸光度上升的程度，从而测算出甲醛的浓度大小。
摘要（英）：

The invention relates to a reagent (kit) for measuring formaldehyde by using an enzyme-multiplied method, an enzyme colorimetric method and an enzyme coupling method as well as a method for measuring the concentration of the formaldehyde, and composition and ingredients of the reagent, belonging to the technical field of food/environmental test. The reagent (kit) comprises the main ingredients of buffer solution, coenzyme, acetyl-coenzyme A, glutathione, formaldehyde dehydrogenase, formic acid C-acetylase, coenzyme A-glutathione reductase and stabilizing agent. The concentration of the formaldehyde is measured by mixing a sample and the reagent according to a certain volume ratio to carry out a series of enzymatic reaction, placing the reactant under an ultraviolet/visible light analyzer and detecting the ascending degree of absorbance at a dominant wavelength of 340nm.

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