The invention discloses a preparation method of a special cooking wine for removing the smell of beef and mutton and enhancing flavor, which is characterized by comprising the following ingredients and preparation process thereof: adding a certain amount of dried mushroom powder and paprika ash powder to water the weight of which is 9-11 times of the total mass of the ingredients, leaching at 90-95 DEG C for 2-4h, and determining the volume after press filtration; adding a certain amount of tangerine peel powder, hawthorn powder, bay leaf powder, fructus amomi powder, cumin powder, pepper powder, tsaoko powder, rhizoma kaempferiae powder, star anise powder, fennel powder, nutmeg powder and clove powder to edible alcohol the weight of which is 3-4 times of the total mass of the ingredients, leaching at room temperature for 15-20d, and determining the volume after press filtration; sequentially mixing a certain amount of trehalose, white granulated sugar, salt, mature vinegar and glutinous rice fermented base wine with the water extract of the ingredients and the alcohol extract of the ingredients; and preparing the special cooking wine with the alcohol content of 15% for removing the smell of beef and mutton and enhancing the flavor by the processes of filtering, filling, sterilizing and the like. The cooking wine can be used for cooking dishes of beef, mutton and the like and processing products thereof, and has obvious effects of removing the smell of beef and mutton and enhancing the flavor.