利索-全球专利检索

  1. 登录
  2. 注册
发明授权
CN101818211B 一种豇豆重花叶病毒的分子检测方法 失效 - 权利终止

一种豇豆重花叶病毒的分子检测方法
摘要：
本发明涉及豇豆重花叶病毒的分子检测方法，属于生物技术领域。该方法包括如下步骤：获得病毒粗提液；制备免疫捕获PCR管；释放免疫捕获PCR管中的豇豆重花叶病毒RNA并以其为模板，以序列为：5’-GGCTTCTGCAGGTGTTCCAA-3’的CPSMVR为引物进行反转录反应，得到反转录产物；以反转录产物为模板，正向引物5’-GGTCAATCCCGGCATTATTG-3’，反向引物CPSMVR，Taq Man探针CPSMVPro 5’FAM-TGTAGCACAATCAGGGCAAACACAGCA-TAMRA 3’，进行实时荧光PCR反应；初始循环数≤35，表示样品感染了豇豆重花叶病毒。
摘要（英）：

The invention relates to a molecule detection method for cowpea severe mosaic virus, which belongs to the technical field of biology. The method comprises the following steps of: obtaining coarse virus extract; preparing an immune capturing PCR tube; releasing cowpea severe mosaic virus RNA in the immune capturing PCR tube and taking the cowpea severe mosaic virus RNA as a template, and performing reverse transcription reaction by using CPSMVR of a sequence 5'-GGCTTCTGCAGGTGTTCCAA-3' as a primer to obtain a reverse transcription product; and performing real-time fluorescent PCR reaction by using the reverse transcription product as a template and using a forward primer 5'-GGTCAATCCCGGCATTATTG-3', a reverse primer CPSMVR and a Taq Man probe CPSMVPro 5'FAM-TGTAGCACAATCAGGGCAAACACAGCA-TAMRA 3', wherein when the initial cycle number is less than or equal to 35, a sample is infected with the cowpea severe mosaic virus.

公开/授权文献
0/0