The invention provides a performance difference detection method of inner and outer asphalt layers of an asphalt film of a hot recycling asphalt mixture, which is characterized by comprising the following steps: step 1: placing the recycling asphalt mixture into a net basket; step 2: soaking the mixture into trichloroethylene solution, standing and then taking out to obtain soak solution 1; step 3: re-soaking the mixture into new trichloroethylene solution, stirring, soaking and then taking out to obtain soak solution 2; step 4: recovering respectively the asphalt in the soak solution 1 and the soak solution 2 to obtain the outer-layer asphalt and the inner-layer asphalt of the asphalt film of the hot recycling asphalt mixture; step 5: taking out the obtained inner-layer asphalt and the outer-layer asphalt for DSR test to obtain G*/sin delta; and step 6: calculating the relative standard deviation RSD of G*/sin delta of the inner and outer asphalt layers. The detection method provided by the invention can effectively solve the problem that it is difficult to accurately evaluate the actual mixing condition of a regenerant or new asphalt and aged asphalt in the hot recycling asphalt mixture of the current asphalt road, thus providing a favorable basis for design and quality assessment of hot recycling process conditions.