An ultrasound diagnosis system and a centesis support controlling method by which positions and directions of a centesis needle traveling to a target region can accurately and easily be determined, including an MPR image data generating unit which generates target image data by setting a prescribed target MPR plane on volume data acquired through 3D scan over a 3D volume including a target region; a centesis needle MPR plane setting unit which generates centesis needle data indicating position and directions of the centesis needle, based on the acquired volume data; a crossing angle detection unit which detects a crossing angle between the target MPR plane and the centesis needle MPR plane including the centesis needle data; and a needling support data generating unit which generates needling support data by composing the target image data and the centesis needle data overlapped with centesis needle image data based on the detected crossin angle.