The invention discloses a corn expression vector containing Hepatitis B virus L gene and application thereof. The method for constructing the corn expression vector comprises the following steps of: 1, designing a pair of primers P1 and P2 according to a coded sequence of the Hepatitis B virus L gene; 2, making the primers P1 and P2 undergo a common chain type polymerase reaction so as to amplifythe Hepatitis B virus L gene; 3, constructing a pCRG-L vector; and 4, constructing a recombinant vector pCAMG-L, and converting agribacterium tumefaciens. In the method, a specific promoter Globulin-1 of the corn seeds, which has double advantages of driving an exogenous gene to express specificity in tissues or organs and realizing high-level expression, is selected, and the fusion gene of the Hepatitis B virus L gene and tubercle Esat6 which is controlled by the specific promoter Globulin-1 of the corn seeds, is constructed, so that the fusion gene is specifically expressed in the corn seeds efficiently. The corn expression vector of the Hepatitis B virus L genes can be applied in the preparation of vaccines.