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发明公开
CN101845509A 狮源性DNA的PCR检测引物、试剂盒及检测方法 失效 - 权利终止 转让

狮源性DNA的PCR检测引物、试剂盒及检测方法
摘要：
狮源性DNA的PCR检测引物、试剂盒及检测方法，本发明分别建立了实时荧光PCR检测和普通PCR检测对狮源性DNA的检测引物、试剂盒以及检测方法。引物序列为SEQ ID NO.1和SEQ ID NO.2；基于此，本发明公开了包含上述引物的狮源性DNA的实时荧光PCR检测试剂盒及相应检测方法和普通PCR检测试剂盒及相应检测方法。本发明的引物特异性强，检测试剂盒及方法简便易用，结果准确，具有很高的特异性和灵敏度。
摘要（英）：

The invention provides a PCR detection primer of Panthera leo DNA, a kit and a detection method thereof, and respectively establishes the PCR detection primer of the Panthera leo DNA, the kit and the detection method thereof by real-time fluorescence PCR detection and common PCR detection, wherein, primer sequences comprise SEQ ID No. 1 and SEQ ID No.2. The invention further discloses the real-time fluorescence PCR assay kit of the Panthera leo DNA containing the primer and the corresponding detection method thereof, the common PCR assay kit and the corresponding detection method thereof. The invention has strong specificity of the primer, simple and convenient detection kit and method with easy use, accurate results, high specificity and high sensitivity.

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