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发明公开
CN101892277A 一种制备抗菌肽的方法及其专用菌株 失效 - 权利终止

一种制备抗菌肽的方法及其专用菌株
摘要：
本发明公开了一种制备抗菌肽的方法及其专用菌株。本发明提供的一种制备抗菌肽的方法，包括如下步骤：1)种子培养：将巴斯德毕赤酵母(Pichia pastoris)GS115/Fow CGMCC No.3814接种到种子培养基中，28℃培养至OD600＝4.0-6.0，离心收集菌体；2)发酵培养：将步骤1)获得的菌体重悬于发酵培养基，再向发酵体系加甲醇，得到抗菌肽，将发酵罐中所有物质记作发酵体系；本发明的实验证明：诱导发酵巴斯德毕赤酵母(Pichia pastoris)GS115/Fow CGMCC No.3814获得抗菌肽对大肠杆菌K88或金黄色葡萄球菌具有明显的抑制作用。
摘要（英）：

The invention discloses an antibacterial peptide preparation method and a specific strain thereof. The antibacterial peptide preparation method provided by the invention comprises the following: 1) a step of seed cultivation, which is to inoculate Pichia pastoris GS115/Fow CGMCC No.3814 into a seed culture medium, cultivate the Pichia pastoris GS115/Fow CGMCC No.3814 at the temperature of 28 DEG C till an optical density at 600nm (OD600) is 4.0 to 6.0 and collect thalli by centrifuging; and 2) a step of fermentation cultivation, which is to heavily suspend the thalli obtained by step 1) in a fermentation medium, add methanol into a fermentation system to obtain an antibacterial peptide and recode all substances in a fermentation tank as the fermentation system. Experiments of the invention prove that the antibacterial peptide obtained by the induced fermentation of the Pichia pastoris GS115/Fow CGMCC No.3814 has an obvious inhibitory effect on colibacillus K88 or staphylococcus aureus.

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