The invention discloses a method for preparing a microbial extracellular enzyme dielectrophoresis sample in finished wheat starter used for making Shao-hsing rice wine, which comprises the following steps of: lixiviating microbial extracellular enzyme in the wheat starter at the low temperature by selecting buffer solution of acetic acid-sodium acetate, precipitating albumen by using pre-cooled acetone solution I containing dithiothreitol and trichloroacetic acid and rinsing a precipitate by using pre-cooled acetone solution containing the dithiothreitol, and finally adding cracking liquid toprepare a high-quality sample which is suitable for dimensional electrophoresis. The method for preparing the microbial extracellular enzyme dielectrophoresis sample in the Shao-hsing rice wine finished wheat starter is quick, reliable, simple and easy to implement, can prepare a dielectrophoresis map with high quality and repeatability for facilitating subsequent analytical identification of albumen by bio-mass spectra, and has a high practical application value.