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CN101906049A 检测血液中肝素含量的荧光探针及合成方法和用途 无效 - 驳回

检测血液中肝素含量的荧光探针及合成方法和用途
摘要：
本发明涉及高选择性、高灵敏度检测肝素，尤其是检测血液中肝素含量的荧光探针及合成方法和用途。将带有取代基R1，R2，R3的三级胺或者带有取代基R1，R2，R3，R4的三级二胺溶解在有机溶剂中，加入卤代烷基芳香烃，其中，卤代烷基芳香烃与带有取代基R1，R2，R3的三级胺或者带有取代基R1，R2，R3，R4的三级二胺的摩尔比值为0.01～50；在反应温度为30～100℃下反应0.5～60小时，将溶液浓缩后，利用硅胶柱层析得到(A)结构或(B)结构的化合物。本发明的荧光探针对肝素具有良好的选择性，本身没有毒副作用，对肝素的检测限可以达到2.0×10-8摩尔/升，适用于血液中肝素的检测。
摘要（英）：

The invention relates to a high-selectivity and high-sensitivity fluorescent probe fordetecting heparin, particularly detecting the heparin content in blood as well as a synthetic method and application. The synthetic method comprises the following steps of: dissolving tertiary amine with substituent groups R1, R2 and R3 or tertiary diamine with the substituent groups R1, R2, R3 and R4 into an organic solvent, and adding halogenated alkyl aromatic, wherein the mol ratio of the halogenated alkyl aromatic to the tertiary amine with the substituent groups R1, R2 and R3 or the tertiary diamine with the substituent groups R1, R2, R3 and R4 is 0.01-50; reacting for 0.5-60 hours at the reaction temperature of 30-100 DEG C, and obtaining a compound with an (A) structure or a (B) structure by utilizing silica gel column chromatography after solution concentration. The fluorescent probe has good selectivity for the heparin, has no toxic or side effect per se, can reach the detection limit for the heparin as 2.0*10<-8> mol/L and is applicable to the detection of the heparin in the blood.

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