The invention discloses a super-resolution fluorescence microscopy method based on tangential polarization, comprising the following steps: carrying out 0-2pi vortex phase coding focus on tangential polarization exciting light, and obtaining an exciting spot below a diffraction limit on a fluorescence sample; adjusting tangential polarization STED laser and the phase coding tangential polarization exciting light to realize confluence and co-axis, focusing on the fluorescence sample to form a circle bread-shaped focusing spot the central point of which coincides with the central point of the exciting spot; adjusting the operating power of the STED laser to cause the area of a luminous point in the exciting spot to reach the super-resolution ratio; and collecting fluorescence emitted from the luminous point and carrying out detection processing to obtain a microimage with the super-resolution ratio. The invention also discloses a device for realizing the super-resolution fluorescence microscopy method based on tangential polarization. In the invention, on the premise of ensuring the super-resolution ratio, the working power of the STED is reduced greatly, thereby lowering bleaching of the sample and avoiding damage for the sample.